DryLab and Empower: a successful combination for the optimisation of mAb subunit analysis
Morgane Lescut
Claire Butré
Arnaud Delobel

The use of retention modelling using DryLab software has the following advantages:

  • No need to know the structure and properties of the molecules to be separated

  • Prediction is based on well-established empirical models.

  • Only a few initial experiments (2-12) need to be performed.Then the separation can be predicted for all conditions.

  • Changes in conditions can be studied in silico (e.g., change of column length, flow rate, gradient time, etc.).

This approach was used in the development of chromatographic methods for the separation of mAb subunits. Nivolumab was
used as a case study. Working at subunit level using an enzyme such as IdeS (FabRICATOR®) allows for a better
chromatographic resolution between the different species (including post-translational modifications) and a better
mass accuracy when MS detection is used.