New contribution to Elsevier publication

New contribution to Carbohydrate Analysis by Modern Liquid Phase Separation Techniques

Quality Assistance has proudly contributed to Elsevier’s publication Carbohydrate Analysis by Modern Liquid Phase Separation Techniques 2^nd edition (978-0-12-821447-3).

Carbohydrate Analysis by Modern Liquid Phase Separation Techniques presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges.

This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques, as it

Covers the basic principles of modern liquid phase separation techniques, along with their applications

Compiles up-to-date information on the field of carbohydrate analysis, along with updates on separation science

Focuses on problems currently faced in carbohydrate analysis and the solutions necessary for further progress

HPLC- and CE-based methods for the characterization of therapeutic glycoproteins

Arnaud Delobel, R&D Director, and Claire I. Butré, R&D Technical Leader, are the authors of Chapter 17 “HPLC- and CE-based methods for the characterization of therapeutic glycoproteins”

Click here to access this publication and chapter now.

1. Introduction

   • Glycosylation of therapeutic proteins and structure/activity relationship

   • Analytical strategies for the characterization of therapeutic glycoproteins

2. Analysis of glycoproteins at the intact and subunit levels

   • Reversed-phase liquid chromatography

   • Hydrophilic-interaction liquid chromatography

   • Ion-exchange chromatography

   • Size-exclusion chromatography

   • Capillary gel electrophoresis

   • Capillary isoelectric focusing

   • Capillary-zone electrophoresis

3. Analysis of proteins at the glycopeptides level

   • Peptide mapping by reversed-phase liquid chromatography

   • Peptide mapping by hydrophilic-interaction liquid chromatography

   • Separation of glycopeptides on porous graphitized carbon (PGC)

   • Capillary electrophoresis

4. Analysis of glycoproteins at the glycan level

   • Strategies for glycan release and derivatization

   • P-LC/MS

   • PGC-LC/MS

   • HPAEC/PAD

   • Hydrophilic-interaction liquid chromatography

   • Mixed-mode chromatography

   • CE/LIF

5. Analysis of glycoproteins at the monosaccharide levels

   • Hydrolysis of glycoproteins into monosaccharides

   • HPAEC/PAD

   • RP-LC

   • CE/LIF

Contact the authors for more information