PEGylated Proteins | Quality Assistance

This study sheet presents specific analytical methods we developed for the characterisation of PEGylated proteins. It covers molecular mass distribution and PEG‑to‑protein ratio determination by MALDI‑TOF mass spectrometry and SEC‑UV/RI/MALS, conjugation site determination by peptide mapping using UPLC‑UV/MS, and free PEG quantification using HPLC with charged aerosol detection.

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