The AID (Autoimmun Diagnostika) MultiSpot reader is a multifunctional imaging device designed for the analysis of enzymatic and fluorescent (FluoroSpot) based ELISpot assays. The device features a high-resolution CCD camera coupled with an automated microscope with multiple objectives, capable of being used with either 96 or 384-well plates.

The unit is controlled with a 21 CFR part 11 compliant software.

The optical design allows for the simultaneous measurement of multiple cytokine secretions at the single-cell level (FluoroSpot assay).

The MultiSpot reader can be used in many research fields such as vaccine development, cancer, allergies, and immune cell characterisation.

ELISpot/FluoroSpot can address the following questions:

• antigen-specific cytokine responses

• antibody-specific secreting cells

• immunomonitoring (vaccine immunogenicity)

• cytotoxic T-cell activity (Granzyme B, IFN-γγ and Perforin released by T cells)